Hello, A comment about how the PCR tracks are displayed that perhaps could use a slight improvement:
I was using the UCSC genome browser to design some experiments today, and noticed something about the PCR feature that I thought was off. Using the PCR tool, I like that you get thick bars indicating where the primers anneal, and a thin region showing additional amplified region. What was not clear to me until now, howe3ver, is that the thick bars are not actually the primers: they seem fixed at 20bp. So, for example, if you use a 22bp primer, the primers' 3'-most 2bp are not displayed as thick. Usually a trivial point, but when carefully designing experiments where the 3' end of the primer must be very precise, it would be nice to have the full primer sequence in a thick dark band. Many thanks! Tim -- Timothy E. Reddy, Ph.D. Postdoctoral Fellow Myers Lab HudsonAlpha Institute for Biotechnology 256-684-0874 (cell) [email protected] _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
