Hello,

I have a bedGraph file,

chr1    4770    4770    1.47749
chr1    4771    4771    2.95498
chr1    4772    4772    2.95498
chr1    4773    4773    2.95498
chr1    4774    4774    2.95498
chr1    4775    4775    2.95498
chr1    4776    4776    2.95498
chr1    4777    4777    5.90996
chr1    4778    4778    4.43247
chr1    4779    4779    4.43247
chrY    57412520        57412520        1.47749
chrY    57412521        57412521        1.47749
chrY    57412522        57412522        1.47749
chrY    57412523        57412523        1.47749
chrY    57412524        57412524        1.47749
chrY    57412525        57412525        1.47749
chrY    57412526        57412526        1.47749
chrY    57412527        57412527        1.47749
chrY    57412528        57412528        1.47749


etc, etc.

it displays just fine when I upload to the UCSC genome Browser.  

I have tried to convert it into a bigWig file using the bedGraphtoBigWig 
command.  The command executes without error,

~/bin/ucsc/bedGraphToBigWig antisense.sorted ~/bin/ucsc/hg18.chrom.sizes  
antisense.bw

When I upload the bigBed file via,

track type=bigWig name="seqc_454_brain_antisense" description="anti-sense seqc 
454 brain alignments (bwa)" 
bigDataUrl=http://physiology.med.cornell.edu/faculty/mason/lab/seqc/seqc_454_brain_antisense.bw


the track loads, but it constantly displayed "no_data", but there should 
certainly be valid data in there, provided that the bedGraph qua bedGraph loads 
just fine!

Any advice?

Thanks a lot,
-Paul


--
Paul Zumbo
Staff Associate
Laboratory of Christopher E. Mason, Ph.D.
Department of Physiology & Biophysics
Weill Cornell Medical College

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