The error message seems to be correct.

33008363 + 2732 + 159
= 33011254
= 33039022 ?  no

It seems that your chromEnd-chromStart
indicates a region that spans more than 30,000 bases.

However your coordinates do not go higher than 2732.

The custom track loader is trying to be helpful
by catching unintended errors.

If you truly do have a very long tail of 30,000
bases that does not align, you will probably have to
ignore that detail and chop the chromEnd
at the end of the last aligning block.

-Galt

2/23/2010 12:47 PM, Bekkari, Kavitha:
> Hi,
> I am trying to load a bed file in to ucsc browser, and I get this error
>
>   Error File 'Dog_CanFam2_RefGene.bed' - Error line 2 of custom track:
> BED blocks must span chromStart to chromEnd. (chromStart +
> chromStarts[last] + blockSizes[last]) must equal chromEnd.
>
> And the second line it is complaining about is provided below, can  you
> please let me know whats wrong with the line?
>
> chr28   33008363        33039022        XM_860454.1     0       +
> 33008363        33039022        0       17
> 201,533,220,194,90,121,90,142,209,86,120,96,192,156,103,179,159,
> 0,201,734,954,1148,1238,1359,1449,1591,1800,1886,2006,2102,2294,2450,255
> 3,2732,
>
>
> Thanks
> Kavitha Bekkari
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