Hello Assif, I need to download for each RefSeq, a sequence of 4000 bases; 2000 upstream the TSS, 2000 downstream from the TSS of all mouse gene. By studying previous mails I get a general idea on how to make it: 1) Start with group=Genes and Gene Prediction Tracks, track=RefSeq Genes and region = genome 2) Select output as "Custom track" and "get output". 3) Choose upstream region 2000 bases and output in Table browser and submit 4) group=Custom Tracks 5) Select output as "Sequence". Name to download file (gzip recommended) and "get output". 6) Now set downstream region as 2000 bases and submit. The zipped fasta file should download.
Is my understanding correct? Besides, I would also like to get a file contain sequence of 0nt upstream of stop codon, 1000nt downstream for each RefSeq from STOP CODON. Would please give me some instructions on how to generate it? Thanks, Yunfei Li -------------------------------------------------------------------------------------- Research Assistant Department of Statistics & School of Molecular Biosciences Biotechnology Life Sciences Building 427 Washington State University Pullman, WA 99164-7520 Phone: 509-339-5096 http://www.wsu.edu/~ye_lab/people.html _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. https://signup.live.com/signup.aspx?id=60969 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
