Hello Derek,

Thank you for sending the image. Everything looks as expected, so this 
is probably just a data interpretation misunderstanding.

To learn about the track and sub-tracks in detail, please see the Method 
section for the track for a very complete description of how the data is 
graphed (click on the track name to see this). At the top of the same 
page, you also have the option to click into the table for the different 
sub-tracks to view the actual data points in the Table browser. A review 
here will allow you to note the values for the data are not negative.

I hope this information is helpful.  Please feel free to contact the
help mailing list again if you require further assistance.

Best regards,
Jen

UCSC Genome Browser Support
http://genome.ucsc.edu/contacts.html
[email protected]  [email protected]

On 7/7/10 11:58 AM, Derek Thibault wrote:
> I had a question about the ENCODE Open Chromatin track, or FAIRE-seq,
> on the UCSC Genome Browser.  It is very obvious where there are peaks
> or apparent regions of open chromatin, and even where the signal
> flatlines and there is no signal.  But i have noticed there are
> regions where there is a pink line that falls below the blue flatline,
> almost like these are regions of "negative" signal.  This is sequence
> data so there should only be two outcomes, either there is sequence or
> there isnt.  So i was wondering what is this pink line?? Ill attach an
> image of an example i found from FAIREseq performed on K562 cells.
> The "negative" signal i am talking about is just upstream of the RBP4
> gene at chromosome location ~95351500.
>
> Thanks for your feedback,
>
> -Derek
>
>
>
>
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