Hello Weldon,

There is not a way to do this within the Genome Browser.  However, you 
could use the stand-alone BLAT program (it is free for academic, 
non-profit, and personal use) to align sequence to whatever reference 
sequence you wish to use.  See the BLAT FAQ page for more details:

http://genome.ucsc.edu/FAQ/FAQblat

Also see the BLAT specification:

http://genome.ucsc.edu/goldenPath/help/blatSpec.html

--
Brooke Rhead
UCSC Genome Bioinformatics Group


On 11/17/08 03:42, Weldon Whitener wrote:
> I'm working on insertions in the human genome, and what I would like to do
> is: take a bit of sequence that contains an insertion and use it as the
> reference that I can then align the human reference back to.  This would
> show the alignment of the reference back to the insertion with the *de
> novo*sequence in between.
> 
> In essence, can I load in my own reference?
> 
> Best
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