Hello again, Guangchao,
Thanks very much for sending your question to the list. I am sorry
that you
were not able to follow the instructions that I gave you yesterday. Let's see
if I can be more helpful today!
When I follow the link from our website for ordering the clone, I am
sent to
this page at the NCBI website:
http://www.ncbi.nlm.nih.gov/genome/clone/orderclone.cgi?db=nucleotide&uid=22713563
Also, you can get the mRNA sequence from our website for the Mammalian
Gene
Collection Full ORF mRNA you are interested in:
>BC037308.1
agcggggcttcctccctctgccttccttccccacggccggccgcctcctc
gcccgcccgcccgcagccgaggagccgaggccgccgcggccgtggcggcg
gagccctcagccatggcctcgggcgacaccctctacatcgccacggacgg
ctcggagatgccggccgagatcgtggagctgcacgagatcgaggtggaga
ccatcccggtggagaccatcgagaccacagtggtgggcgaggaggaggag
gaggacgacgacgacgaggacggcggcggtggcgaccacggcggcggggg
cggccacgggcacgccggccaccaccaccaccaccatcaccaccaccacc
acccgcccatgatcgctctgcagccgctggtcaccgacgacccgacccag
gtgcaccaccaccaggaggtgatcctggtgcagacgcgcgaggaggtggt
gggcggcgacgactcggacgggctgcgcgccgaggacggcttcgaggatc
agattctcatcccggtgcccgcgccggccggcggcgacgacgactacatt
gaacaaacgctggtcaccgtggcggcggccggcaagagcggcggcggcgg
ctcgtcgtcgtcgggaggcggccgcgtcaagaagggcggcggcaagaaga
gcggcaagaagagttacctcagcggcggggccggcgcggcgggcggcggc
ggcgccgacccgggcaacaagaagtgggagcagaagcaggtgcagatcaa
gaccctggagggcgagttctcggtcaccatgtggtcctcagatgaaaaaa
aagatattgaccatgagacagtggttgaagaacagatcattggagagaac
tcacctcctgattattcagaatatatgacaggaaagaaacttcctcctgg
aggaatacctggcattgacctctcagatcccaaacaactggcagaatttg
ctagaatgaagccaagaaaaattaaagaagatgatgctccaagaacaata
gcttgccctcataaaggctgcacaaagatgttcagggataactcggccat
gagaaaacatctgcacacccacggtcccagagtccacgtctgtgcagaat
gtggcaaagcttttgttgagagttcaaaactaaaacgacaccaactggtt
catactggagagaagccctttcagtgcacgttcgaaggctgtgggaaacg
cttttcactggacttcaatttgcgcacacatgtgcgaatccataccggag
acaggccctatgtgtgccccttcgatggttgtaataagaagtttgctcag
tcaactaacctgaaatctcacatcttaacacatgctaaggccaaaaacaa
ccagtgaaaagaagagagaagacccttctcgaccacgggaagcatcttcc
agaagtgtgattgggaataaatatgcctctcctttgtatattatttctag
gaagaattttaaaaatgaatcctacacacctaagggacatgttttgataa
agtagtaaaaattaaaaaaaaaaaactttactaagatgacattgctaaga
tgctctatcttgctctgtaatctcgtttcaaaaacacagtgtttttgtaa
agtgtggtcccaacaggaggacaattcatgaacttcgcatcaaaagacaa
ttctttatacaacagtgctaaaaatgggacttcttttcacattcttataa
atatgaagctcacctgttgcttacaatttttttaattttgtattttccaa
gtgtgcatattgtacacttttttggggatatgcttagtaatgctacgtgt
gatttttctggaggttgataactttgcttgcagtagattttctttaaaag
aatgggcagttacatgcatacttcaaaagtattttcctgtaaaaaaaaaa
aaagttatataggttttgtttgctatcttaattttggttgtattctttga
tgttaacacattttgtataattgtatcgtatagctgtattgaatcatgta
gtatcaaatattagatgtgatttaatagtgttaatcaatttaaacccatt
ttagtcactttttttttccaaaaaaatactgccagatgctgatgttcagt
gtaatttctttgcctgttcagttacagaaagtggtgctcagttgtagaat
gtattgtaccttttaacacctgatgtgtacatcccatgtaacagaaaggg
caacaataaaatagcaatcctaaagcaaaaaaaaaaaaaaa
Regards,
----------
Ann Zweig
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu
Guangchao Sui wrote:
> Hello,
>
> I was kindly helped by Ann and she would like me to send my future questions
> to the the genome mailing list .
>
> I am trying to order a clone that contains the promoter of human YY1 gene.
> When I followed the instruction from Ann to search for the "YY1 clone", I did
> find the BC037308. However, I think this clone was derived from mRNA (ORF).
> Am I right?
>
> Even we assume this clone was a fragment of GENOMIC DNA, how can I know
> whether it contains both the YY1 "gene" and the "promoter" region, because I
> don't think the sequence of the clone is provided?
>
> I apologize if the questions are too naive.
>
> Many thanks for your time,
>
> Guangchao
>
> __________________________________
> Guangchao Sui, Ph. D.
> Assistant Professor
> Department of Cancer Biology
> Wake Forest University School of Medicine
> Hanes 4052
> Medical Center Boulevard
> Winston-Salem, NC 27157
> Phone: 336-713-0052
> Fax: 336-716-0255
> E-mail: [EMAIL PROTECTED]
>
>
> ________________________________
>
> From: Ann Zweig [mailto:[EMAIL PROTECTED]
> Sent: Wed 11/19/2008 6:39 PM
> To: Guangchao Sui
> Subject: Re: UCSC Genome Bioinformatics.
>
>
>
> Hello Guangchao,
>
> Yes, you can use the UCSC Genome Browser to find what you want. I
> will walk
> you through it. Start at the page you mentioned in your email, then press the
> "Genome Browser" link. This will take you to a gateway page where you can
> choose the organism you are interested in. It looks like you're interested in
> human, so just leave the defaults as they are. Type into the
> "position/search"
> box: YY1 -- then press the submit button. This will display a list of all
> genes
> and mRNAs that match this search term. Choose the gene you're interested in
> (probably the first one in the list) and follow the link for that gene. Now
> you
> are looking at your gene in the Genome Browser.
>
> Note that there are several annotation tracks for this section of the
> human
> genome. One of the tracks is from the Mammalian Gene Collection (it is
> green).
> It looks like the Gene in that track that most closely fits the YY1 gene you
> are interested in is: BC037308. Click directly on the image of that gene to
> see
> many details of the MGC Gene. This details page includes more information
> about
> this gene as well as the clone source. Of particular interest to you will be
> the "Order MGC clone" link. Follow that link to learn how to order the clone.
>
> I hope this information is helpful to you.
>
> In the future, please direct your questions to the genome mailing
> list at
> [email protected] -- our moderated forum for user questions and discussion.
> You will likely get a quicker response to your question.
>
>
> Regards,
>
> ----------
> Ann Zweig
> UCSC Genome Bioinformatics Group
> http://genome.ucsc.edu <http://genome.ucsc.edu/>
>
> Please feel free to search the Genome mailing list archives by visiting our
> home
> page, clicking on "Contact Us", then typing a word or phrase into the search
> box. On that same page
> (http://genome.ucsc.edu/contacts.html), you can subscribe to the Genome
> mailing
> list.
>
>
>
>> ---------- Forwarded message ----------
>> From: *Guangchao Sui* <[EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>>
>> Date: Wed, Nov 19, 2008 at 1:50 PM
>> Subject: UCSC Genome Bioinformatics.
>> To: [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>
>>
>>
>> Hi, Dr. Zhu,
>>
>>
>>
>> I have some questions in using the http://genome.ucsc.edu/index.html
>> website.
>>
>>
>>
>> I am trying to find and order a DNA clone that contains the genomic DNA
>> fragment with the promoter sequence of human YY1. A friend of mine
>> recommended "the UCSC Genome Bioinformatics". However, when I visit your
>> website, I just don't know how to get what I want.
>>
>>
>>
>> If you could provide me some hints to find what I want, I will very much
>> appreciate. Otherwise, please let me know who is the person I should
>> contact.
>>
>>
>>
>> Thanks and best regards,
>>
>>
>>
>> Guangchao
>>
>> __________________________________
>>
>> Guangchao Sui, Ph. D.
>>
>> Assistant Professor
>>
>> Department of Cancer Biology
>>
>> Wake Forest University School of Medicine
>>
>> Hanes 4052
>>
>> Medical Center Boulevard
>>
>> Winston-Salem, NC 27157
>>
>> Phone: 336-713-0052
>>
>> Fax: 336-716-0255
>>
>> E-mail: [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>
>>
>>
>>
>>
>
>
>
> _______________________________________________
> Genome maillist - [email protected]
> http://www.soe.ucsc.edu/mailman/listinfo/genome
_______________________________________________
Genome maillist - [email protected]
http://www.soe.ucsc.edu/mailman/listinfo/genome
