Just download BLAT and use it locally.
Much faster.

-Galt


On Tue, 20 Jan 2009, Grille, James wrote:

> I have 173 pages of 100mer DNA sequences (25 per page) I need to BLAT.
> Does this mean I can only submit one page of 25 every 6.3 minutes (and
> thus 19hrs to complete)?
>
> -Jim
>
> -----Original Message-----
> From: Pauline Fujita [mailto:[email protected]]
> Sent: Tuesday, January 20, 2009 12:35 PM
> To: Grille, James {CSD.~Tucson}
> Cc: [email protected]
> Subject: Re: [Genome] large batch queries
>
> Hello James,
>
> Please see this previously answered mailing list question regarding our
> policy on usage restrictions:
>
> http://www.soe.ucsc.edu/pipermail/genome/2005-October/008881.html
>
> If you let us know what you are trying to retrieve from our site we can
> recommend a way for you to obtain the data without exceeding our limits.
>
> Best regards,
>
> Pauline Fujita
> UCSC Genome Bioinformatics Group
> http://genome.ucsc.edu
>
>
> Grille, James wrote:
>> I received this message and am writing to you because I do indeed have
>> large queries I am trying to submit.:
>>
>>
>>
>> There is a very high volume of traffic coming from your site (IP
> address
>> 74.202.188.254) as of Mon Jan 19 09:26:04 2009 (California time). So
>> that other users get a fair share of our bandwidth, we are putting in
> a
>> delay of 10.1 seconds before we service your request. This delay will
>> slowly decrease over a half hour as activity returns to normal. This
>> high volume of traffic is likely due to program-driven rather than
>> interactive access, or the submission of queries on a large number of
>> sequences. If you are making large batch queries, please write to our
>> [email protected] public mailing list and inquire about more
> efficient
>> ways to access our data. If you are sharing an IP address with someone
>> who is submitting large batch queries, we apologize for the
>> inconvenience. Please contact [email protected] if you think
> this
>> delay is being imposed unfairly.
>>
>>
>>
>> What can be done?  Can you contact me ASAP?
>>
>>
>>
>> James Grille
>> Scientist, Molecular Probe Core Team
>>
>> Ventana Medical Systems, Inc.
>> a member of the Roche Group
>> 1910 E. Innovation Park Drive
>> Tucson, Arizona 85755
>>
>> Tel: +1 520 229 4191
>>
>> mailto: [email protected]
>> www.ventanamed.com
>>
>>
>> Confidentiality Note: This message is intended only for the use of the
>> named recipient(s) and may contain confidential and/or proprietary
>> information. If you are not the intended recipient, please contact the
>> sender and delete this message. Any unauthorized use of the
> information
>> contained in this message is prohibited.
>>
>>
>>
>> _______________________________________________
>> Genome maillist  -  [email protected]
>> http://www.soe.ucsc.edu/mailman/listinfo/genome
>>
>
>
> _______________________________________________
> Genome maillist  -  [email protected]
> http://www.soe.ucsc.edu/mailman/listinfo/genome
>
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