hi Jim, Have you checked if someone else at your company has already installed blat locally? That would certainly be your easiest option. Please note that there is a license fee for commercial downloads/installs of blat: http://www.kentinformatics.com/products.html.
-Donna ------------ Donna Karolchik UCSC Genome Browser Project Manager http://genome.ucsc.edu Galt Barber wrote: > Just download BLAT and use it locally. > Much faster. > > -Galt > > > On Tue, 20 Jan 2009, Grille, James wrote: > >> I have 173 pages of 100mer DNA sequences (25 per page) I need to BLAT. >> Does this mean I can only submit one page of 25 every 6.3 minutes (and >> thus 19hrs to complete)? >> >> -Jim >> >> -----Original Message----- >> From: Pauline Fujita [mailto:[email protected]] >> Sent: Tuesday, January 20, 2009 12:35 PM >> To: Grille, James {CSD.~Tucson} >> Cc: [email protected] >> Subject: Re: [Genome] large batch queries >> >> Hello James, >> >> Please see this previously answered mailing list question regarding our >> policy on usage restrictions: >> >> http://www.soe.ucsc.edu/pipermail/genome/2005-October/008881.html >> >> If you let us know what you are trying to retrieve from our site we can >> recommend a way for you to obtain the data without exceeding our limits. >> >> Best regards, >> >> Pauline Fujita >> UCSC Genome Bioinformatics Group >> http://genome.ucsc.edu >> >> >> Grille, James wrote: >>> I received this message and am writing to you because I do indeed have >>> large queries I am trying to submit.: >>> >>> >>> >>> There is a very high volume of traffic coming from your site (IP >> address >>> 74.202.188.254) as of Mon Jan 19 09:26:04 2009 (California time). So >>> that other users get a fair share of our bandwidth, we are putting in >> a >>> delay of 10.1 seconds before we service your request. This delay will >>> slowly decrease over a half hour as activity returns to normal. This >>> high volume of traffic is likely due to program-driven rather than >>> interactive access, or the submission of queries on a large number of >>> sequences. If you are making large batch queries, please write to our >>> [email protected] public mailing list and inquire about more >> efficient >>> ways to access our data. If you are sharing an IP address with someone >>> who is submitting large batch queries, we apologize for the >>> inconvenience. Please contact [email protected] if you think >> this >>> delay is being imposed unfairly. >>> >>> >>> >>> What can be done? Can you contact me ASAP? >>> >>> >>> >>> James Grille >>> Scientist, Molecular Probe Core Team >>> >>> Ventana Medical Systems, Inc. >>> a member of the Roche Group >>> 1910 E. Innovation Park Drive >>> Tucson, Arizona 85755 >>> >>> Tel: +1 520 229 4191 >>> >>> mailto: [email protected] >>> www.ventanamed.com >>> >>> >>> Confidentiality Note: This message is intended only for the use of the >>> named recipient(s) and may contain confidential and/or proprietary >>> information. If you are not the intended recipient, please contact the >>> sender and delete this message. Any unauthorized use of the >> information >>> contained in this message is prohibited. >>> >>> >>> >>> _______________________________________________ >>> Genome maillist - [email protected] >>> http://www.soe.ucsc.edu/mailman/listinfo/genome >>> >> >> _______________________________________________ >> Genome maillist - [email protected] >> http://www.soe.ucsc.edu/mailman/listinfo/genome >> > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
