Dear UCSC Team,
i would like to get your comment about a common problem about differently value
between the sequence cutting in variable regions and the result of
Electrophoresis-Gels with restriction enzymes!
I have a problem on such region at Chr. 16q21.
The region sequence which can be exact cut by EcoRI
(http://rna.lundberg.gu.se/cutter2/) in 7,859kb in size.
63,909,639-63,917,498=7859bp
But the result of my electrophoreses with radioactive marked sequence (marker
8, see the photo by attachment data) showed that the wild type allele by germen
population (by 131 persons) is located by about 10kb!!(2kb longer)
I know that this region is very variable (fragile site 16B is located in this
region and contain variable number of tandem repeats) but how you can explain
this variant results (about 2kb different) between the sequence analysis and
the reality? Is it a problem by any variable regions? I mean I expected a wild
type allel by about 7.8kb but I get a DNA- fragment with about 10 kb in size?
How is possible?
best regards from Germany
Javad Karim Zad HaghPhD-Student
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