Dear Javad, Unfortunately we do not have the resources to investigate our users' scientific inquiries on a case-by-case basis. Additionally, interpretation of experimental results is best left up to the researcher.
We do have a restriction enzymes track in the Genome Browser, under "Mapping and Sequencing Tracks". Perhaps viewing this track alongside some of our repeat tracks could shed some light on your situation. Best of luck to you. If you have any questions about how to use the Genome Browser, please don't hesitate to contact us again. Kayla Smith UCSC Genome Bioinformatics Group ----- "Javad Karim Zad Hagh" <[email protected]> wrote: > Dear UCSC Team, > > i would like to get your comment about a common problem about > differently value between the sequence cutting in variable regions > and the result of Electrophoresis-Gels with restriction enzymes! > > I have a problem on such region at Chr. 16q21. > The region sequence which can be exact cut by EcoRI > (http://rna.lundberg.gu.se/cutter2/) in 7,859kb in size. > > 63,909,639-63,917,498=7859bp > > But the result of my electrophoreses with radioactive marked sequence > (marker 8, see the photo by attachment data) showed that the wild type > allele by germen population (by 131 persons) is located by about > 10kb!!(2kb longer) > I know that this region is very variable (fragile site 16B is located > in this region and contain variable number of tandem repeats) but how > you can explain this variant results (about 2kb different) between the > sequence analysis and the reality? Is it a problem by any variable > regions? I mean I expected a wild type allel by about 7.8kb but I get > a DNA- fragment with about 10 kb in size? How is possible? > > best regards from Germany > Javad Karim Zad HaghPhD-Student > > > > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
