Hello Rudy: There are a couple of ways to retrieve sequence from a list of genes. One is here, in a previously answered mailing list question: https://lists.soe.ucsc.edu/pipermail/genome/2007-April/013248.html
And here is our FAQ on retrieving batch sequence from an assembly: http://genome.ucsc.edu/FAQ/FAQdownloads#download32 As for your numerical concerns, please have a look at this wiki page (with attention to the procedures listed at the bottom): http://genomewiki.ucsc.edu/index.php/Gene_Set_Summary_Statistics I hope this helps to get you started. Please don't hesitate to contact us again if you require further assistance. Kayla Smith UCSC Genome Bioinformatics Group ----- "Rudy Spangler" <[email protected]> wrote: > I want to design pcr assays for a list of human refseq genes ... i > want to design the assays around large introns where convenient ... > optimally around large introns nearest the 3'-end of the transcript > ... can i get the exon sequences (including 5' and 3' UTRs) together > with a number giving the size of the introns for each gene in the > list? > > thanks > > rudy spangler > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
