Hi Alan, The hg18 human assembly has two tracks that contain miRNA data from which you can obtain the sequence of miR-17 with its promoter region, sno/miRNA and RNA Genes.
Here is a quick guide to get the sequence you are looking for. From the Gateway page (http://genome.ucsc.edu/cgi-bin/hgGateway), select the Mar 2006 (NCBI36/hg18) human assembly. In the box labeled "position or search term", delete the position, enter "miR-17", and click the submit button. The first result listed should be under wgRna (the sno/miRNA track), click the link "hsa-mir-17 at chr13:90800860-90800943". This will take you to the browser image and you'll see a red item labeled hsa-mir-17. Click on that item to see the details. From the details page, you can select "View DNA for this feature" and from here you can select "Promoter/Upstream by 1000 bases". You can adjust the number of bases upstream by replacing "1000" with a number of your choice. Click "get DNA" and you'll see the sequence. I hope this answer was helpful. Please don't hesitate to contact the mail list again if you have any further questions. Katrina Learned UCSC Genome Bioinformatics Group Alan Griffith wrote, On 09/17/10 11:08: > To whom it may concern > > hi there, i was hoping for some clarity on using the UCSC website to find > out sequence information for miR-17, the sequence for this i can obtain > easily however i cannot get the sequence upstream (ie promoter region) of > the RNA, how do i go about it using the website?? Ive tried with the various > tabs on the site but find it hard to get any suitable answer! any help at > all would be greatly appreciated, the sequence for mouse or rat or human > would do?? > > Regards > Alan Griffith > NICB > Dublin City University > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
