Hello, The RefSeq Gene ATP7B does indeed align to the negative strand, which is why you only have the option from the RefSeq Genes page to get DNA from the negative strand.
If you need reverse-complemented sequence, another way to get it would be from the "Get DNA" link. From the main Genome Browser page (http://genome.ucsc.edu/cgi-bin/hgTracks), hit the "DNA" link in the blue bar at the top of the page. It will default to the forward strand. You can use the "extended case/color options" to highlight the result based on RefSeq Genes. -- Brooke Rhead UCSC Genome Bioinformatics Group On 10/03/10 03:23, gulnaz shabir wrote: > Dear Sir/Madam" > I want to get the reference sequence of the gene ATP7B so that i can > detect > any mutations in my experimental samples after comparing with it .Whenever i > search its sequence with "hg19"refGene > location chr13:52506806-52586630 > i receive sequence of exons ,introns and promoter on -strand or reverse > strand .but i think it should be on + strand or forward strand. > after searching for DNA sequence on+strand, i could not get the boundries for > exons,introns and promoter region .why is this so? > i am trying hard to get its sequence . > pl answer me what should i do? > > > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
