Hi Manisha, From the BLAT web site description:
"Multiple sequences may be searched if separated by lines starting with '>' followed by the sequence name." "Only DNA sequences of 25,000 or fewer bases and protein or translated sequence of 10000 or fewer letters will be processed. Up to 25 sequences can be submitted at the same time. The total limit for multiple sequence submissions is 50,000 bases or 25,000 letters." Also, instead of pasting in the sequences, you can also upload a file containing the sequences separated with a '>'. Please also see this FAQ about using BLAT for large batch queries: http://genome.ucsc.edu/FAQ/FAQblat.html#blat2 Please don't hesitate to contact the mail list again if you have any further questions. Katrina Learned UCSC Genome Bioinformatics Group Manisha Sajnani wrote, On 10/8/2010 2:50 AM: > Respected Sir > > Currently we are using BLAT at UCSC site. We are interested to find > homology search with our sequences and we have around 5lac sequences with us > to be uploaded for BLAT. Does Blat provide any option for batch upload? We > want to blat using following parameter: > 1.Taking reference genome -Cow > 2.Assembly: Oct 2007 > 3.Query type: BLAT guess > 4.Source Output : Query, Score > 5.Output type : psl > > This is quite urgent . Please provide us with the solution as soon as > possible > Thanking you, > > Dr. Manisha Sajnani > Research Fellow > Department of Animal Biotechnology > Anand Agricultural University > Anand, Gujarat > India. > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
