Hi Pete,

The UCSC Genes process is somewhat complex, with mRNAs and ESTs 
contributing evidence to exons and introns, but not being used 
exclusively as evidence for a gene.  The methods (which can be different 
for different assemblies, depending on when the track was made) are 
outlined on the description pages.  To get to the methods, click on the 
blue "UCSC Genes" track name for the assembly you are looking at
(for hg19, the page is: 
http://genome.ucsc.edu/cgi-bin/hgGene?hgg_do_kgMethod=1&db=hg19).

If you have already seen this page and you are asking for more 
specifics, please feel free to contact us again at [email protected].

--
Brooke Rhead
UCSC Genome Bioinformatics Group


On 10/25/10 09:48, Pete Shepard wrote:
> Dear Genome Browser,
> 
> I am wondering for your known genes track what is the requirement for a 3'
> end. I understand that it is based on a number of different sources but If
> it is based on ESTs, Do you require the ESTs to have a polya tail and if so,
> how many As? If it is based solely on mRNA, is a polya tail required and if
> so, how many As?
> 
> Thanks
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