Good morning, I am interested in GPSM1 on human chr. 9. Before beginning gene expression experiments, I have been researching the 3 different RefSeq transcripts on the UCSC Browser. According to the GenBank entry, transcript variant NM_015597.4 is derived exclusively, save one bp, from cDNA clone (mRNA) BC009979.1. However, I am unable to locate any ESTs matching the unique 3' end of BC009979.1 by BLASTn (of dbEST), despite the existence that I noted of a putative polyadenylation signal ATTAAA close to the end of the the BCOO9979.1 sequence. I attempted to access the details of this mRNA from the website in the cited 2002 PNAS article (PMID12477932) but parts of that website appear to be down. I am curious about the support for the true existence of this transcript and would really appreciate any help you could give me.
Sincerely, Amy Dietrich Amy Dietrich Doctoral Candidate, Dept. of Human and Molecular Genetics Virginia Commonwealth University Virginia Institute for Psychiatric and Behavioral Genetics _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
