Hello Dr. Purnick, You may be interested in our restriction enzymes track(found in the "Mapping and Sequencing" track group):
http://genome.ucsc.edu/cgi-bin/hgTrackUi?&c=chr12&g=cutters You can read more about how the track is constructed by clicking on the blue/gray bar to the left of the track in the main display or by clicking on the track title above the drop down menu. If you know the coordinates of your region of interest you can simply input them into the position/search box in the Mouse (mm9) browser. Otherwise if you have sequence from your region interest you can use our BLAT tool to map your sequence to the mouse genome: http://genome.ucsc.edu/cgi-bin/hgBlat Hopefully this information was helpful and answers your question. If you have further questions or require clarification feel free to contact the mailing list at [email protected]. Regards, Pauline Fujita UCSC Genome Bioinformatics Group http://genome.ucsc.edu On 01/12/11 14:07, Priscilla Purnick wrote: > Greetings, > > I need to choose restriction enzymes to digest my genomic (mouse) DNA such > that I can determine in a Southern blot the difference between a genomic > gene and a gene that has been inserted via lentiviral transduction. In > order to do this I need to know: > > 1. Size of the fragment containing the genomic gene after cutting with the > enzyme(s). > 2. If I can find an enzyme that cuts in an intron region of the genomic gene > and what the size of those fragments would then be. > > If anyone knows of anything else I need to look for and how to do it with > this browser (for these types of Southerns), that would be great. I know > this is probably a very basic question, however, I am a new user of this > browser, so any help would be appreciated. > > Thank you all in advance! > Dr. Priscilla Purnick > Please respond to: [email protected] > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
