Hello Angie & Vanessa,

thank you for your helpful reply.

One more question, the link that I assume lists the available types
http://genome.ucsc.edu/cgi-bin/das/hg19/types
does not seem to work. I tried it on Mac using Chrome and Safari.

Best wishes,
Thomas

On Fri, Jan 14, 2011 at 19:05, Angie Hinrichs <[email protected]> wrote:
> Hi Thomas,
>
> We actually do have a simple DAS server:
>
> http://genome.ucsc.edu/cgi-bin/das/hg19/features?type=ensGene;segment=13:32889611,32973347
> http://genome.ucsc.edu/cgi-bin/das/hg19/features?type=knownGene;segment=13:32889611,32973347
> http://genome.ucsc.edu/cgi-bin/das/hg19/features?type=refGene;segment=13:32889611,32973347
> http://genome.ucsc.edu/cgi-bin/das/hg19/types
>
> (segment=chr13 works too, but we accept "chr"-less coords for compatibility 
> with Ensembl)
>
> In general, our DAS server shows a simple GFF view of the data; more complete 
> info can be obtained by FTP (hgdownload.cse.ucsc.edu), Table Browser, or 
> mysql (genome-mysql.cse.ucsc.edu), especially for the UCSC Genes (knownGene) 
> track with many related database tables.
>
>> Also, are the genomic coordinates different between UCSC, Ensembl and
>> NCBI?
>
> It depends on which genomic coordinates we're talking about.  The same genome 
> assembly sequence is used by all 3: hg19 = GRCh37, obtained from NCBI.  
> Genomic coordinates of annotations may differ, for example if we show Ensembl 
> Genes from a previous release of Ensembl, although we do regularly update 
> those.  Also, our RefSeq Genes track is based on our own BLAT alignments of 
> cDNA sequences downloaded from GenBank, so there may be some small 
> differences in coords vs. NCBI.
>
> Hope that helps,
> Angie
>
> ----- "Vanessa Kirkup Swing" <[email protected]> wrote:
>
>> From: "Vanessa Kirkup Swing" <[email protected]>
>> To: "Thomas Juettemann" <[email protected]>
>> Cc: [email protected]
>> Sent: Thursday, January 13, 2011 2:59:03 PM GMT -08:00 US/Canada Pacific
>> Subject: Re: [Genome] Intron/Exon boundaries
>>
>> Dear Thomas,
>>
>> For newbie questions, the archived mailing list questions are a great
>> resource! To get to this from the home page click on "Contact", enter
>> a search term for "Search the Genome mailing list archives:" and click
>> on "submit".
>>
>>
>> Here is a previously answered mailing list question that should help
>> you:
>>
>> https://lists.soe.ucsc.edu/pipermail/genome/2009-April/018677.html
>>
>>
>> Information on the hg19 (GRCh37) assembly can be found on the gateway
>> page:
>>
>> http://genome.ucsc.edu/cgi-bin/hgGateway?org=Human&db=hg19
>>
>> The hg19(GRCh37) assembly was created by The Genome Reference
>> Consortium
>> (http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/)and is used
>> by UCSC, Ensembl and NCBI.
>>
>>
>> Hope this helps! Please contact the mailing list if you have further
>> questions.
>>
>> Vanessa Kirkup Swing
>> UCSC Genome Bioinformatics Group
>>
>>
>>
>> ----- Original Message -----
>> From: "Thomas Juettemann" <[email protected]>
>> To: [email protected]
>> Sent: Thursday, January 13, 2011 6:38:25 AM
>> Subject: [Genome] Intron/Exon boundaries
>>
>> Hello all,
>>
>> again one of those newbie questions.
>>
>> I was wondering if you could give me some background information
>> about
>> intron/exon boundaries.
>> I am looking for something on UCSC site that is similar to this:
>>
>> http://www.ensembl.org/das/Homo_sapiens.GRCh37.transcript/features?segment=13:32889611,32973347
>>
>> It seems to be possible by using the table browser, but is there also
>> a way using DAS or via FTP?
>>
>> Also, are the genomic coordinates different between UCSC, Ensembl and
>> NCBI?
>>
>>
>> Thank you & Happy New Year!
>> Thomas
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