Hi Javeed,

We added links to the page, as you suggested.  Scroll down to:

start -- The start of the aligning region in the source sequence. This 
is a zero-based number. If the strand field is '-' then this is the 
start relative to the reverse-complemented source sequence (see 
Coordinate Transforms).

Please let us know if you think they would be helpful in a different 
location!  And thank you for the suggestion.

--
Brooke Rhead
UCSC Genome Bioinformatics Group


On 01/31/11 09:46, Jaaved Mohammed wrote:
> Hi Hiram,
> 
> Thanks for pointing out this wiki. Since the MAF format description on the
> USCS browser help-page does not make this interpretation explicit (see
> http://genome.ucsc.edu/FAQ/FAQformat.html#format5), may I suggest to put a
> link to this wiki there? I think it would benefit lots of folks out there.
> 
> Thanks,
> Jaaved
> 
> -----Original Message-----
> From: Hiram Clawson [mailto:[email protected]] 
> Sent: Monday, January 31, 2011 12:11 PM
> To: Jaaved Mohammed
> Cc: [email protected]
> Subject: Re: [Genome] Insect MAF file coordinate-sequence disagreement
> 
> See also:
> 
> http://genomewiki.ucsc.edu/index.php/Coordinate_Transforms
> 
> Brian Raney wrote:
>> We realize that these coordinate systems can be confusing, but using
>> these systems internally makes it easier to avoid different code for
>> alignments on the different strands.
>>
>> On Thu, Jan 27, 2011 at 2:15 PM, Jaaved Mohammed <[email protected]>
> wrote:
>>> Hello,
>>>
>>> I am seeing numerous discrepancy between the sequences and coordinates in
>>> the insect 15-way multiple-alignment files
>>> (http://hgdownload.cse.ucsc.edu/goldenPath/dm3/multiz15way/) primarily
> for
>>> D. ananassae and D. virilus. Hopefully I can explain this as concisely as
>>> possible, however, let me know how I can clarify:
> 
> _______________________________________________
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