Hello David,

We do not have a single tool to append data to bigWig files as you 
describe, however, there is an easy way to accomplish this with existing 
utilities. Simply extract the data from your big* file (i.e. with 
bigWigToBedGraph) merge the resulting extracted file with your new data 
(i.e. you could merge the resultant bedgraph file with a new bedgraph 
file) and then convert back to bigWig using bedGraphToBigWig.

Note that this utility (bedGraphToBigWig) will require that at most one 
value is listed for each base pair. The reason for this is because 
bigWig keeps track of summary information at different resolutions so it 
doesn't have to look at each base pair when looking at large chrom 
ranges. To calculate these zoom levels, the bigWig builder needs to look 
at all the data.

Best regards,

Pauline Fujita
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu

On 02/09/11 06:05, Davide Cittaro wrote:
> Hi all, 
> I'm extensively using bigWig format for ChIP-seq data. I usually have a 
> single bedgraph I can convert to a bigWig using witToBigWig executable. This 
> said, I may have in the future the need to merge multiple dataset and/or 
> writing bigWig at different times (i.e. append data to an old bigWig). Is 
> this possible with current binaries (and current bigWig implementation, of 
> course)?
> 
> Thanks
> 
> d
> /*
> Davide Cittaro, PhD
> 
> Cogentech - Consortium for Genomic Technologies
> via adamello, 16
> 20139 Milano
> Italy
> 
> tel.: +39(02)574303007
> e-mail: [email protected]
> */
> 
> 
> 
> 
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome

_______________________________________________
Genome maillist  -  [email protected]
https://lists.soe.ucsc.edu/mailman/listinfo/genome

Reply via email to