Hi David,

This previous mailing list question should be able to help you:

https://lists.soe.ucsc.edu/pipermail/genome/2005-March/006938.html

I hope this information is helpful.  Please feel free to contact the 
mail list again if you require further assistance.

Best,
Mary
------------------
Mary Goldman
UCSC Bioinformatics Group

On 2/8/11 2:37 PM, David Lyon wrote:
> 1944    NM_005649    chr5    -    178138521    178157703    178139060    
> 178156023    5    178138521,178152376,1
> 78153999,178155990,178157556,    
> 178140622,178152472,178154126,178156074,178157703,    0    ZNF354A    cmpl    
> cmpl    1,1,0,0,-1,
>
>
> In the above example the gene NM_005649 is located on the negative strand and 
> so why is the transcriptional start site listed as "178138521" should it not 
> be "178157703" ? Since this gene is located on the -ve strand and thus 
> transcription should occur in the 5 -->  3 direction on the negative strand,  
> therefore should transcription start at "178157703"
>
>
> Or is there something I am missing?
>
> Thanks
>
>
>
>
>
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