Good Afternoon Janeela:

To confirm the measurements of phastCons and the question of alignment in
any particular location, open up the alignment track displays and view
the actual aligned bases to get a definite decision on the actual
conservation for any particular location.

You may also find it useful to examine phastCons and phyloP conservation
prediction tracks on the human Feb. 2009 (GRCh37/hg19) assembly in
the 46-way conservation track.  Please note the discussion of the
meanings of phastCons and phyloP predictions:

http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&c=chr21&g=cons46way

Hopefully this information was helpful and answers your question. If you
have further questions or require clarification feel free to contact the
mailing list at genome at soe.ucsc.edu.

--Hiram

janeela khan wrote:
>  Dear all,
> I am looking at the conservation of certain positions/regions  using the 
> PhastConsElements28way, I got a score=0 and lod=132 for one of the position, 
> I am interested in knowing, what does this score means? I have read 
> http://compgen.bscb.cornell.edu/phast/phastCons-HOWTO.html to understand the 
> idea behind  but it doesnot seems sufficeint. please guide me.
> My interest is to see which of the positions are highly conserved in my data.
> Thanks in advance,
> Best janeela,                                           
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