hi guys: this is probably a really stupid question, but it do confuse me...
based on the description of SpilcedEST track: the genomic sequence between EST alignment blocks must be at least 32 bases in length and have GT/AG ends. and by a simply checking, i find that substantial proportion of distances between 2 neighboring alignment blocks of an EST sequence is less or equal than 1, doesn't this mean these 2 neighboring blocks are actually continuous region? if they are, why would them be 2 blocks? by the description, it sounds like alignment blocks are exons... or i misunderstand these blocks are just produced by Blat, and i need to check canonical GT/AT sites from genome sequence in order to get right exon-intron structures? Thanks~~ _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
