Dear Madam/Mr,
I am a postgraduate of Wuhan
university in China. I'm writing to ask for the detailed processesof mouse
cDNA library construction.As we know,mouse EST database come from sequencing of
the cDNA library ,but people use different methods to construct the mouse cDNA
library .What were the concrete approaches you adopted here? I
downloaded the mouse EST database from UCSC genome browser for my study .When I
analyze the special ESTs I concern,I find some ESTs are chimeric,which may
be produced by templete switch of reverse transcriptase when synthetising the
first strand of cDNA.So,I need to know the detailed processes you constructed
the cDNA library,will you do me a favor?I'm looking forward to your response.
yours,sincerely.
Wang jun.
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