Hello Wang jun, You can see the methods that were used to create the mouse EST tracks in the Genome Browser. Go to http://genome.ucsc.edu/cgi-bin/hgGateway, select "mouse" from the drop-down menu, and hit "submit". Scroll down to the "mRNA and EST Tracks" section, then click on the "Mouse ESTs" link. Scroll down to the methods section to read about how the track was constructed.
If you have further questions, please feel free to contact us again at [email protected]. -- Brooke Rhead UCSC Genome Bioinformatics Group On 06/11/11 06:23, [email protected] wrote: > Dear Madam/Mr, > I am a postgraduate > of Wuhan university in China. I'm writing to ask for the detailed processesof mouse cDNA library construction.As we know,mouse EST database come from sequencing of the cDNA library ,but people use different methods to construct the mouse cDNA library .What were the concrete approaches you adopted here? I downloaded the mouse EST database from UCSC genome browser for my study .When I analyze the special ESTs I concern,I find some ESTs are chimeric,which may be produced by templete switch of reverse transcriptase when synthetising the first strand of cDNA.So,I need to know the detailed processes you constructed the cDNA library,will you do me a favor?I'm looking forward to your response. > yours,sincerely. > Wang jun. > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
