Thank you for your response, Mary.
Kathleen

On Tue, Jun 14, 2011 at 7:00 PM, Mary Goldman <[email protected]> wrote:
> Hi Kathleen,
>
> I'm a bit unsure of your first question but I think you are asking if the
> probes in hgFixed.gnfAtlas2All and hg19.knownToGnfAtlas2 will have the same
> chromosomal location? The answer is yes, they should map to the same
> chromosomal location.
>
> You can see the chromosomal coordinates of all the probes listed in
> hgFixed.gnfAtlas2All in the table hg19.gnfAtlas2. From there, you can
> determine if there is a gene that reasonably fits those coordinates.
>
> I hope this information is helpful.  Please feel free to contact the mail
> list again if you require further assistance.
>
> Best,
> Mary
> ------------------
> Mary Goldman
> UCSC Bioinformatics Group
>
> On 6/14/11 8:49 AM, kathleen askland wrote:
>>
>> Hello Luvina,
>>
>> A couple more follow-up questions:
>>
>> Following your instructions, below, I was able to obtain the table
>> that matched the hg19 Probe name (i.e., variable name
>> 'hg19.knownToGnfAtlas2.value') to gene symbol (i.e.,
>> 'hg19.kgXref.geneSymbol') via hg19 UCSC id (i.e., variable name
>> 'hg19.knownToGnfAtlas2.name'). (I'll refer to this table as the
>> UCSCid-ProbeID-Gene mapping table.)
>>
>> However, I am using the all replicates expression data from the
>> hgFixed database and wanted to know:
>> 1) Will all probes identically named in both the hg19 and hgFixed
>> datasets are, in fact, mapped to the same chromosomal position (such
>> that the hg19 probe-gene mapping obtained in the UCSCid-ProbeID-Gene
>> mapping table would hold for the identical probe ID in the hgFixed
>> expression data table)?
>> 2) For probes that are in the hgFixed dataset but NOT in the above
>> hg19 UCSCid-ProbeID-Gene mapping table (e.g., 1053_at), is there any
>> data table available in the UCSC genome browser that would provide the
>> correct chromosomal map and gene symbol?
>>
>> Thank you so much for your valuable assistance.
>>
>> Kathleen
>>
>>
>>
>> On Fri, Jun 10, 2011 at 6:31 PM, Luvina Guruvadoo<[email protected]>
>>  wrote:
>>>
>>> Hi Kathleen,
>>>
>>> You will first need to retrieve UCSC IDs from the hg19.knownToGnfAtlas2
>>> table, then use hg19.kgXref to retrieve the corresponding gene names. I
>>> suggest you do this using the Table Browser. Make the following
>>> selections:
>>>
>>> table: knownToGnfAtlas2
>>> output format: selected fields from primary and related tables
>>>
>>> Click 'get ouput'. On the following page, select the 'name' and 'value'
>>> fields, scroll down and select 'hg19 kgXref' and click 'Allow Selection
>>> From
>>> Checked Tables'. From here, you can select 'geneSymbol' then click 'get
>>> output'.
>>>
>>> I hope this helps. Please contact us again at [email protected] if you
>>> have any further questions.
>>> ---
>>> Luvina Guruvadoo
>>> UCSC Genome Bioinformatics Group
>>>
>>>
>>>
>>> kathleen askland wrote:
>>>>
>>>> Hello,
>>>> I have an additional question pertaining to the GNF Atlas 2 expression
>>>> data available through UCSC.
>>>> If I download the hgFixed.gnfHumanAtlas2All table, and join the
>>>> hg19.kgXref.geneSymbol field from the hg19.kgXref table, will the
>>>> matching of probe to gene symbol be correct even though the two tables
>>>> are from different databases?
>>>> Thank you,
>>>> Kathleen
>>>>
>>>>
>>>>
>>>
>>
>>
>



-- 
Kathleen Askland, MD
Assistant Professor
Department of Psychiatry & Human Behavior
Warren Alpert School of Medicine
Brown University/Butler Hospital

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