Hi:
I was trying to find the conserved regions in human 3'UTR and 5'UTR. I have downloaded the multiple sequence alignment of primate. I was examining the file, say, chr1.phyloP46way.primate.wigFix. It is my understanding that each value corresponds to the probability of an nt being conserved at a given position. That is, it must vary from 0 to 1 while 1 being highly conserved. But examining the file, it has negative value such as -2.34 etc... I am not sure on how to interpret the phastCons value in the file.. I scanned through UCSC help or user's forum. Some others also have similar questions, but no answer. Any help??? Thanks Tom Thomas Randall, PhD Bioinformatics Scientist, Contractor National Institute of Environmental Health Sciences P.O. Box 12233, Research Triangle Park, NC 27709 [email protected]<mailto:[email protected]> 919-541-2271 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
