+1:  http://www.mail-archive.com/[email protected]/msg03607.html


-----Original Message-----
From: [email protected] [mailto:[email protected]] On 
Behalf Of daniel.gaffney
Sent: Wednesday, September 28, 2011 11:01 AM
To: [email protected]
Subject: [Genome] PhyloP off by one errors?

Hi,
I've been trying to convert the hg18 phyloP scores for placental mammals to 
bigWig format and I've come across what look
like numerous off-by-one errors (and others) in the files posted here:

ftp://hgdownload.cse.ucsc.edu/goldenPath/hg18/phyloP44way/placentalMammals/

I've been using wigToBigWig from the UCSC source to do the conversion. I use 
the following code:

zcat $FILE | wigToBigWig -clip stdin chromSizes.txt test.bw

where FILE is a wig file from the above directory. So far I've found the 
following errors:

There's more than one value for chr1 base 220000001 (in coordinates that start 
with 1).
There's more than one value for chr13 base 60000001 (in coordinates that start 
with 1).
There's more than one value for chr16 base 20000001 (in coordinates that start 
with 1).
There's more than one value for chr2 base 60000001 (in coordinates that start 
with 1).
There's more than one value for chr3 base 180000001 (in coordinates that start 
with 1).
There's more than one value for chr4 base 110000001 (in coordinates that start 
with 1).
There's more than one value for chrY base 20000001 (in coordinates that start 
with 1).

Removing the value line before the wig line e.g.:

0.561   <==== delete this line
fixedStep chrom=chr1 start=220000001 step=1

seems to fix the problem, but its pretty time-consuming. I hope this is of use 
to somebody - but any ideas why its happening?
Best
Daniel



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