Hi Malcolm,
Thanks for the update - does it seem odd to you that both our examples show
that problems occur in the lines preceding a block
whose start coordinate is "....00001"? Could this be an 0/1 error in array
indexing perhaps?
Best
Daniel

On Wed, Sep 28, 2011 at 7:40 PM, Cook, Malcolm <[email protected]> wrote:

> +1:  http://www.mail-archive.com/[email protected]/msg03607.html
>
>
> -----Original Message-----
> From: [email protected] [mailto:[email protected]] On
> Behalf Of daniel.gaffney
> Sent: Wednesday, September 28, 2011 11:01 AM
> To: [email protected]
> Subject: [Genome] PhyloP off by one errors?
>
> Hi,
> I've been trying to convert the hg18 phyloP scores for placental mammals to
> bigWig format and I've come across what look
> like numerous off-by-one errors (and others) in the files posted here:
>
> ftp://hgdownload.cse.ucsc.edu/goldenPath/hg18/phyloP44way/placentalMammals/
>
> I've been using wigToBigWig from the UCSC source to do the conversion. I
> use the following code:
>
> zcat $FILE | wigToBigWig -clip stdin chromSizes.txt test.bw
>
> where FILE is a wig file from the above directory. So far I've found the
> following errors:
>
> There's more than one value for chr1 base 220000001 (in coordinates that
> start with 1).
> There's more than one value for chr13 base 60000001 (in coordinates that
> start with 1).
> There's more than one value for chr16 base 20000001 (in coordinates that
> start with 1).
> There's more than one value for chr2 base 60000001 (in coordinates that
> start with 1).
> There's more than one value for chr3 base 180000001 (in coordinates that
> start with 1).
> There's more than one value for chr4 base 110000001 (in coordinates that
> start with 1).
> There's more than one value for chrY base 20000001 (in coordinates that
> start with 1).
>
> Removing the value line before the wig line e.g.:
>
> 0.561   <==== delete this line
> fixedStep chrom=chr1 start=220000001 step=1
>
> seems to fix the problem, but its pretty time-consuming. I hope this is of
> use to somebody - but any ideas why its happening?
> Best
> Daniel
>
>
>
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