Hi, YU This page has the info you want:
http://hgwdev.cse.ucsc.edu/cgi-bin/hgPcr Forward Primer - Must be at least 15 bases in length. Reverse Primer - On the opposite strand from the forward primer. Minimum length of 15 bases. Min Perfect Match - Number of bases that match exactly on 3' end of primers. Minimum match size is 15. Min Good Match - Number of bases on 3' end of primers where at least 2 out of 3 bases match. -Galt 10/21/2011 6:10 PM, Yuan Jian: > Dear UCSC support, > how many mismatchs are allows in UCSC In-Silico PCR? > > thanks > YU > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
