Chih-Ying Lin wrote:
HI
1. Would you please tell me the reason that "generally people use NaCl
or KCl in the solution with protein"?
2. Why does PDB file of the protein NOT Carrying ions?
PDB structures do not contain information about the properties of the solvent or
precipitant used to obtain the structure. Occasionally some additive (glycerol,
etc.) will be present since it is bound to the protein. Some background on
X-ray crystallography or NMR will explain structure determination.
-Justin
Thank you
Lin
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--
========================================
Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
========================================
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