Hi HCP users,

I’m trying to run probtrackX on some HCP data, can someone provide some 
guidance on how to run this using surfaces? I’m following the FSL user guide 
but they often refer to files which are absent from the HCP data as far I can 
tell. (e.g., "and also that you have a file called struct.nii.gz that you have 
used as an input to FreeSurfer recon_all program."

Basically, I’d like to define subcortical seeds and track to cortical surfaces.

For the subcortical seed regions I can easily create binary masks to use as 
seeds using fslmaths based on intensity values from the freesurfer LUTs. I 
could do the same with the cortical regions but I have read that tracking to 
cortical regions is best done with surfaces instead of volumes.

So, when selecting “classification targets” can I simply add in a cifti file? 
(e.g., 100307/T1w/aparc+aseg.nii.gz OR 100307/T1w/aparc.a2009s+aseg.nii.gz)** 
and then tell probtrackX to output the matrices I want?

**I assume using the files in the T1w dir is best because these are in the same 
space as the diffusion data and therefore don’t require transformation.

On a different note, if I select a surface file as a seed instead, it asks for 
a “surface reference image” for Freesurfer. Which file is best used for this 
purpose?

Cheers,
Linden

——————————————————————

Linden Parkes

PhD Candidate
Monash Clinical & Imaging Neuroscience (MCIN)
School of Psychological Sciences
Monash Biomedical Imaging
Monash University

A: 770 Blackburn Rd, Clayton, 3168, Vic, Australia
T: +61 3 9902 9435  |  0402 516 574
E: [email protected]
W: www.med.monash.edu.au/psych/mcin/


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