Potentially.   Presumably your DWI show up “upside-down” in the A/P axis when 
viewed in ‘fslview’?

You could use ‘fslreorient2std’ to change to LAS (or RAS) orientation on your 
already converted NIFTI, but you’ll need to adjust the bvecs appropriately as 
well.  Or, try another DICOM converter (e.g., dcm2niix) to see if that 
generates LAS orientation by default with your data.

I’d try that on 1-2 subjects for which you are having issues, and see if it 
solves your problem.

cheers,
-MH

--
Michael Harms, Ph.D.
-----------------------------------------------------------
Conte Center for the Neuroscience of Mental Disorders
Washington University School of Medicine
Department of Psychiatry, Box 8134
660 South Euclid Ave. Tel: 314-747-6173
St. Louis, MO  63110 Email: [email protected]

From: Maarten Vaessen <[email protected]<mailto:[email protected]>>
Date: Wednesday, October 19, 2016 at 8:47 AM
To: Michael Harms <[email protected]<mailto:[email protected]>>
Cc: "[email protected]<mailto:[email protected]>" 
<[email protected]<mailto:[email protected]>>
Subject: Re: [HCP-Users] alignment issues with diffusion data

The data is from a 2d EPI sequence converted from DICOM with mrtrix routines.
From mri_info I get:

ras xform present
    xform info: x_r =  -1.0000, y_r =   0.0000, z_r =   0.0000, c_r =     3.8554
              : x_a =   0.0000, y_a =  -0.9285, z_a =  -0.3714, c_a =    -7.2492
              : x_s =   0.0000, y_s =  -0.3714, z_s =   0.9285, c_s =    15.6468
Orientation   : LPS
Primary Slice Direction: axial

The issue is then with the DICOM conversion?

Thx,

-Maarten





On Wed, Oct 19, 2016 at 3:39 PM, Harms, Michael 
<[email protected]<mailto:[email protected]>> wrote:

Is your DWI data by any chance saved with an orientation other than LAS or RAS?

--
Michael Harms, Ph.D.
-----------------------------------------------------------
Conte Center for the Neuroscience of Mental Disorders
Washington University School of Medicine
Department of Psychiatry, Box 8134
660 South Euclid Ave.Tel: 314-747-6173<tel:314-747-6173>
St. Louis, MO  63110Email: [email protected]<mailto:[email protected]>

From: 
<[email protected]<mailto:[email protected]>>
 on behalf of Maarten Vaessen <[email protected]<mailto:[email protected]>>
Date: Wednesday, October 19, 2016 at 6:50 AM
To: "[email protected]<mailto:[email protected]>" 
<[email protected]<mailto:[email protected]>>
Subject: [HCP-Users] alignment issues with diffusion data

Hello experts,

I have been trying to use the HCP Diffusion preprocessing pipeline for my own 
diffusion data. However, I run into a lot of issues at the final stage of the 
pipeline where the dwi data is registered to the T1w_acpc space (the freesurfer 
part of the pipeline runs fine on the structural images, the eddy correction 
also seems to run fine).
So far I have not had a single subject where this alignment went smoothly and 
was accurate.
It seems to be a problem with flirt. In the DiffusiontoStructural.sh subscript 
there are several calls to flirt (or epi_ref_dof) with the T1 as reference and 
for some reason flirt gives very wrong results like AP inverted or cerebellum 
to frontal lobe matching etc...
I have managed to solve some cases with a call to only flirt and not 
epi_reg_dof and adding -useqform to the flirt options, but some I only managed 
by doing a rough manual alignment first. When the initial reg to T1 in ok-ish 
the bbregister and later parts all work very well.
I don't think my dwi data is very special (1.5 mm^3 90 dir 2 shell), so I have 
no idea what might be the issue here or how to solve it (in a consistent way).

-Maarten

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