Hi Lesley:
There is a fairly large literature on this subject. I my opinion, most
of the fancier stains are not worth the effort and the results are
disappointing. In a separate mailing, I will send you some references.
Geoff
Lesley Bechtold wrote:
Hi Histonetters,
If anyone has a good protocol for staining plastic sections, could they share it?
We have samples embedded in an epoxy resin for TEM but they love the semi-thick
sections on the glass slide and asked if we could do something other than a
toluidine blue O, the standard stain for plastic. We have tried H&E and PAS
with no success. If there some way to treat the sections in order to get them to
stain? Is there another stain we could try?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
[email protected]
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