I use McNeal as a substitute for H&E. I use it on MMA embedded bone.
It is to intense (for us) to be used on JB-4. I think you might need to
try a few different stains to select the best.
*/ /*
*/-- You are what you do - not what you say --/*
*/Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
3525 John Hopkins Ct. 0863
San Diego, CA 92121-08630
858-822-1251 voice
858-534-5304 fax
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Walters, Katherine S wrote:
Perhaps you could try the Paragon stain. It looks more like an H&E.
Kathy Walters
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Lesley
Bechtold
Sent: Wednesday, September 30, 2009 10:12 AM
To: [email protected]
Cc: Pete Finger
Subject: [Histonet] staining of plastic sections
Hi Histonetters,
If anyone has a good protocol for staining plastic sections, could they
share it? We have samples embedded in an epoxy resin for TEM but they
love the semi-thick sections on the glass slide and asked if we could do
something other than a toluidine blue O, the standard stain for plastic.
We have tried H&E and PAS with no success. If there some way to treat
the sections in order to get them to stain? Is there another stain we
could try?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)
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