Rena, we use butylacetat for clearing and before coverslipping with Pertex (xylolbased, resinous). Butylacetat tolerates ethanol-carryover more than xylene, but evapourates very fast after couverslipping. But is it possible, that butylacetat or Pertex itself cause the fading?
Do you think coverslipping with a waterbased medium is a good alternative? Gudrun -----Ursprüngliche Nachricht----- Von: Rena Fail [mailto:[email protected]] Gesendet: Samstag, 21. November 2009 00:15 An: [email protected]; [email protected] Betreff: Re: [Histonet] rhodanin stain fading Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail ----- Original Message ---- From: Gudrun Lang <[email protected]> To: [email protected] Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
