Thank you for answering, I use the right compound, but as a lazy girl I wrote the short name. But you are absolutely right, precision should also be important with the terminology in histotechnic.
Next time I will try the aqueous mounting media. I took the procedure from your book, but did the mistake to exchange the mounting media. Gudrun Lang _____ Von: John Kiernan [mailto:[email protected]] Gesendet: Sonntag, 22. November 2009 07:33 An: Rena Fail Cc: [email protected]; [email protected] Betreff: Re: [Histonet] rhodanin stain fading Rena is right. Published methods prescribe aqueous mounting media. This probably is the most sensitive histochemical staining method for detecting copper. See Irons et al 1977 Arch. Path. Lab. Med. 101:298-301. The histochemical reagent for copper is not rhodanine (which exists but would not stain copper deposits) or rhodanin (which does not exist). It is paradimethylaminobenzylidenerhodanine. It's indexed as a D in the catalogues of vendors of chemicals. There is no short name for this reagent and technique. This is not a pedantic gripe. You need to have the right compound and also the right instructions for the technique. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Rena Fail <[email protected]> Date: Friday, November 20, 2009 18:15 Subject: Re: [Histonet] rhodanin stain fading To: [email protected], [email protected] > Alcohol will cause the stain to fade, even the small amt. that > is carried over to the xylene over the course of the day. Make > sure your xylene is fresh and coverslip immediately after staining > Rena Fail > > > ----- Original Message ---- > From: Gudrun Lang <[email protected]> > To: [email protected] > Sent: Fri, November 20, 2009 2:16:49 PM > Subject: [Histonet] rhodanin stain fading > > Hi! > > Yesterday I did a rhodine stain for copper. Immediatly after > staining I saw > not many but distinct red granula in hepatocytes. > > I am new to this stain, so I was happy (and a little bit proud), > that it had > worked. > > I also stained one slide over night for comparison. Today > morning the first > stained slides has faded and not the smallest bit of red colour > could be > seen. > > > > Is this a common problem? What causes the fast fading? > > > > Gudrun > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
