Susan, If you are talking about frozen sections of fixed tissue, they do not adhere well to slides (even charged slides) no matter how thick or thin. I'm not sure why you are treating the sections with alcohol chloroform either. That is pretty harsh treatment and totally unnecessary for Nissl staining.
Why not stain the sections free-floating and then mount them from xylene? Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Truscott, Tom Sent: Friday, July 29, 2011 10:22 AM To: Michael, Susan; [email protected] Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem to have trouble holding on to very thick sections. Another possibility is too much glacial acetic acid in your working solution of cresyl-too much acid can loosen the bonds on the plus slides. Tom T -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 6:12 AM To: [email protected] Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
