We have done the same, but also adding a 50 and 70 ETOH as well. Our slides are also gel coated. We also have them in a 1 to 1 alcohol/chloroform, but only have them in for 30 minutes.
Laura -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 6:12 AM To: [email protected] Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
