I would look at the antibody dilution and redo your titer study, your primary antibody is probably too concentrated.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jason McGough Sent: Tuesday, January 31, 2012 12:32 PM To: [email protected] Cc: Janna Hope Subject: [Histonet] Factor XIIIa background staining problems We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried many different protocols (i.e. enzyme pretreatment, high and low pH pretreatment, different incubation times, etc.)and nothing seems to fix this background staining. Our vendor told us that the dilution is good but we are questioning the actual dilution or the shelf life of the antibody. The antibody is a long ways from reaching the expiration date but we are still questioning it's stability. Has anybody else experienced this or know a solution? Thanks, Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
