Hi, Think of yourself as a reference lab as well. You have a validated protocol for one clone already. Validate the new clone against the current. Your medical director can help you determine how many cases are sufficient. Depending on the antibody we validate anywhere from five cases to twenty. In my opinion, this in-house validation is the best way. The only variable is the clone. Your processing, staining platform, detection...all stays the same. Good luck melissa
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 8:51 AM To: [email protected] Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | [email protected] | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
