Outside reference (any other lab using an antibody to the same target) are helpful for those antibodies/cases for which you have scant material and may need more data. Then you can send your slides to them for staining, and stain some of their slides, and maybe trade controls.
Tim Morken -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 9:05 AM To: Morken, Timothy; [email protected] Subject: [Histonet] RE: IHC Validation Thank you all for the info. I agree that in-house validation is the way to go. The only reason we had been using an outside reference lab was due to recommendations during a JC inspection which were applied to our area, but not really meant for IHC. The inspector was not a histology person, so it was a little difficult to get our point across. But they were adamant that with only one instrument the slides should be sent out for comparison. Thanks everyone! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | [email protected] | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Morken, Timothy [mailto:[email protected]] Sent: Friday, May 04, 2012 12:02 PM To: Dessoye, Michael J; [email protected] Subject: RE: IHC Validation All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) [email protected] -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 5:51 AM To: [email protected] Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | [email protected] | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
