That artifact cannot be fixation related because if there is some part of the sample that is well fixed is the outer limit, where the NBF enters. It does not matter what stainer you use, they all can deliver incorrect amounts of reagents. René J.
________________________________ From: Caroline Miller <[email protected]> To: "Manfre, Philip" <[email protected]> Cc: Rene J Buesa <[email protected]>; Mary Benoit <[email protected]>; "[email protected]" <[email protected]> Sent: Tuesday, September 24, 2013 11:13 AM Subject: Re: [Histonet] edge effect on IHC However, I use the Shandon sequenza, and I still see edge effect. I always thought it was a fixation related. Maybe even that the edge staining is 'more real' than the rest!! Histology, it's all voodoo!! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 24, 2013, at 7:51 AM, "Manfre, Philip" <[email protected]> wrote: > I concur with Rene'. That was my first thought. Edges drying up on an > autostainer due to hydrophobia or insufficient amounts of solution. Stainers > usually have the option to adjust the volume that is applied and sometimes > even the location on the slide (zones). Thorough deparaffinization is also > essential to avoid the hydrophobic repelling of solutions and wash buffers. > Also be sure your reagents have some form of surfactant or detergent > (e.g.Tween 20) in them to break the surface tension and allow the even spread > of whatever is being applied. > > Phil. > > > Philip Manfre, B.A., HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP45-251 > PO Box 4 > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > [email protected] > > > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Rene J Buesa > Sent: Tuesday, September 24, 2013 10:36 AM > To: Mary Benoit; [email protected] > Subject: Re: [Histonet] edge effect on IHC > > Sometimes that "edge effect" is caused by drying when there is not enough > reagents. If you are using an automated stainer, please check the amount of > delivered reagents. > René J. > > > ________________________________ > From: Mary Benoit <[email protected]> > To: "[email protected]" <[email protected]> > Sent: Monday, September 23, 2013 5:00 PM > Subject: [Histonet] edge effect on IHC > > > I have been aware of some "edge effect" on an occasional slide, but lately we > noticed the effect is showing up in the area of the slide that is farthest > from the labels. We use DAKO Link Autostainer and their pretreatment baths > for everything. One case in particular we stained a large melanoma met to > colon that was well fixed and processed that stained only the edge, however > when we sent block to Neogenomics reference lab, their slide stained evenly > over the entire tumor area. We both used a melanoma cocktail. Not sure what > is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > [email protected] > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
