Hey Mary,
I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which uses a vortex type of mixing. Good luck! Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > From: Mary Benoit <[email protected]> > Subject: [Histonet] edge effect on IHC > To: "[email protected]" > <[email protected]> > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=us-ascii > > I have been aware of some "edge effect" on an occasional slide, but lately we > noticed the effect is showing up in the area of the slide that is farthest > from the labels. We use DAKO Link Autostainer and their pretreatment baths > for everything. One case in particular we stained a large melanoma met to > colon that was well fixed and processed that stained only the edge, however > when we sent block to Neogenomics reference lab, their slide stained evenly > over the entire tumor area. We both used a melanoma cocktail. Not sure what > is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
