+1 to Linda, but I have found no difference on overnight vs multiple days. Fatty liver is hard to do on all counts! It is tough enough sometimes to get a decent section on the slide.
Thanks for the other suggestions, certainly something I would try in the future Yours Caroline Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 15, 2015, at 5:07 PM, Linda Prasad (SCHN) > <[email protected]> wrote: > > Usually with the fatty tissues, I pick them up on superfrost slides and let > it air dry for 2-3 days at room temperature and then perform the ORO stains. > So far they seem to stay on. > > Linda Prasad | Senior Scientist | Histopathology > t: (02) 9845 3306 | f: (02) 9845 3318 | e: [email protected] | > w: www.schn.health.nsw.gov.au > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > Locked Bag 4001, Westmead 2145, NSW Australia > > ♲ Please consider the environment before printing this email. > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Jo-Ann Bader, > Ms. > Sent: Thursday, 16 April 2015 1:40 AM > To: [email protected] > Subject: [Histonet] ORO tissue falling off > > We are having difficulty with a particulate set of very, very fatty mouse > livers. The normal livers from this set stay on the slides the fatty livers > fall off. We have used different types of charged slides and we have even > tried to drench the charged slides in Stay-On, dry them and then put the > frozen tissues on (despirate times call for despirate measures). No luck > Does anyone have any other ideas. Help Help > > Jo-Ann Bader > Histology Coordinator > Goodman Cancer Research Center > 1600 Pine Ave. W, > Room 312 > Montreal Quebec, H3A 1A3 > Email: [email protected]<mailto:[email protected]> > Office Tel: 514-398-5647 > Lab: Tel: 514-398-8270 > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Sydney Children's > Hospitals Network. > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Sydney Childrens Hospital's > Network accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
