I used to be the Queen of ORO in my lab at the University of Arizona. I had some of the fattiest livers that could be possible from different research projects. I never had a problem with the livers staying on the slide. I used Epic coated slides or Stat Lab slides and the protocol was from Freida's second edition. The stain was from PolyScientific R&D. I cut the sections at 5 microns, let them sit in the cryostat for about 30 minutes and then fixed in 37% Formaldehyde for about 10-30 minutes - never really timed it. All steps of staining were done gently, one slide at a time, no matter how many slides I had to stain. Sections were beautiful - wish I could post a picture here. Retired now - sometimes I miss doing things like this.
Andi Grantham ________________________________________ From: [email protected] [[email protected]] on behalf of Linda Prasad (SCHN) [[email protected]] Sent: Wednesday, April 15, 2015 5:07 PM To: 'Jo-Ann Bader, Ms.'; [email protected] Subject: [Histonet] RE: ORO tissue falling off Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: [email protected] | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, 16 April 2015 1:40 AM To: [email protected] Subject: [Histonet] ORO tissue falling off We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help Jo-Ann Bader Histology Coordinator Goodman Cancer Research Center 1600 Pine Ave. W, Room 312 Montreal Quebec, H3A 1A3 Email: [email protected]<mailto:[email protected]> Office Tel: 514-398-5647 Lab: Tel: 514-398-8270 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. *********************************************************************************
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