Hi Histonetters! I’m gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I’ve found a protocol used by the Children’s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf
Two things: Iodine crystals are out of the question. Can I replace that with Lugol’s iodine solution or should I just substitute Weigert’s for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain. There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it? Thanks! Angela -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
