Angela Lamberth at the La Jolla [California] Institute for Allergy & Immunology asks:
>>I'm gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I've found a protocol used by the Children?s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf Two things: Iodine crystals are out of the question. Can I replace that with Lugol's iodine solution or should I just substitute Weigert's for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain? There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it?<< THE expert on the Movat pentachrome stain is Akemi Allison, a very senior histotechnologist who's published about it. I can't remember if she's on Histonet, but you can contact her on Facebook - I hear from her there quite often. I'm not personally familiar with the stain, but I don't think either of those substitutions will work. You need to find out more about saffron before you work with it, because it's so expensive. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
