Hi there Histonetters!
I am currently working through some issues of unprocessing tissue to then
restain 'whole mount' with various stains, and then reprocess back to
I am running into issues of tissue being brittle and scratchy tissue after
reprocessing and sectioning.
Can anyone offer any advice on kinder dewaxing procedures and / or
additives to the unprocessing / processing fluids that might help in the
resulting piece of tissue?
I have currently been putting it through the cleaning cycle on the
processor, so standard xylene then alcohol treatment.
thanks in advance for your advice!
Caroline Miller (mills)
Director of Histology
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